MraY

=__MraY__=

MraY is an integral membrane protein, comprised of 10 transmembrane alpha-helices, which is present in low numbers in wild type cells (Winn et al, 2010). There are only three strictly conserved aspartate residues (Asp-115; Asp-116; Asp-267) which might function in the active site, and these are all located on the cytoplasmic loops of the membrane (see image below).
 * __Structure:__**



__**Enzyme Kinetics:**__ E. coli MraY has been over-expressed, extracted into detergent micelles and characterised kinetically using a continuous fluorescence assay. However, further purification was not possible (Winn et al, 2010)